MOLEKBASE is a freely available database layout in Access as well as a data conversion and filtering tool primarily developed to store and work with tri-digit microsatellite data. Information for up to 25 codominant loci can be stored. Data can be filtered according to country of origin, species, population, sampling year as well as individuals and transformed into Genepop, SpaGeDi, Structure, Baps and Convert input formats.. A paper for Molecular Ecology Resources is in preparation. Standard disclaimer This program is provided "as-is". The authors disclaim all warranties, expressed or implied, regarding the performance of this software. You may use, copy, modify and redistribute MOLEKBASE as long as its source is acknowledged and no fee is charged. If you have any questions, problems or suggestions don’t hesitate to contact me at marjana.pucko@gozdis.si. How to use MOLEKBASE When you first open the Access database layout you will find some samples there belonging to three different populations. They are there as an example file. When you have learnt how MOLEKBASE works, please replace them with your own samples. Place your data into the Access database layout. You can do this by either entering the data directly or importing it from Excel (which I think is easier). Use tri digit number format: relative sizes in bp or codes. Do not change the order of the predefined columns. Add the columns you wish to at the end. When you have stored your data in the database, open the exe file. Filtering and conversion form will open. You can switch between Slovenian and English language with the button in the upper left hand corner. Select the data you want to work with using Boolean operators. Make sure to use brackets correctly. Option “only active samples” (the samples you have inactivated in Access are left out) has a built in “and” operator. Expression “Population 1 or population 2 + only active samples” will return all samples from population 1 and only active samples from population 2, while expression “(population 1 or population 2) + only active samples” will return only active samples in both populations. After filtering expression is assembled, click Refresh button. You can now hide the filter by clicking Show Filters button. You will see the filtered data appear in the upper window. Go through it to see, whether brackets as well as “or” and “and” operators have been used correctly. If you make a mistake in the filtering expression, use a double click to delete it. Transform the data into required input format by clicking the button on the right. And don’t lose your temper if you have to wait a minute. If you have a large data set, it can take a while. Once the transformed data appears, copy it to clipboard, open Notepad and paste it there. Name and save it. Between transforming the data into another input format please click Clear button. Data is always sorted according to population code. Good luck with the analysis.